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flag tag and part of the multiple cloning site  (Promega)

 
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    Structured Review

    Promega flag tag and part of the multiple cloning site
    Flag Tag And Part Of The Multiple Cloning Site, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag+tag+and+part+of+the+multiple+cloning+site/flag+tag+and+part+of+the+multiple+cloning+site/pm15175277-204-11-17
    Average 90 stars, based on 1 article reviews
    flag tag and part of the multiple cloning site - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Construct:

    Article Title: Occupancy and synergistic activation of the FMR1 promoter by Nrf-1 and Sp1 in vivo.
    Article Snippet: .. To construct pACTINFL, the FLAG tag and part of the multiple cloning site were PCR-amplified from pCMV-tag2b (Promega) using 50-CGGTTAACGCCACCATGGATTACAAGGATGACG-30 and 50-CCGTCTAGACTTGATATCGAATTCCTGCAGCCC -30 and inserted into the pACTIN vector (63), a gift from Dr Paul Wade, using the Hpa I and Xba I sites. .. To construct pACTINFL-Nrf-1, Nrf-1 was PCR-amplified from a pC MVtag2b vector containing Nrf-1 inserted at the BamHI and Hind III sites (pCMV-Nrf-1) using 50 -TCTGGATCCATGGAGGAACACGGAGTG ACCCAAACCGAA-30 and 50-C GACTCGAGTCACTGTTCCAATGTCACCACCTCC-30.

    FLAG-tag:

    Article Title: Occupancy and synergistic activation of the FMR1 promoter by Nrf-1 and Sp1 in vivo.
    Article Snippet: .. To construct pACTINFL, the FLAG tag and part of the multiple cloning site were PCR-amplified from pCMV-tag2b (Promega) using 50-CGGTTAACGCCACCATGGATTACAAGGATGACG-30 and 50-CCGTCTAGACTTGATATCGAATTCCTGCAGCCC -30 and inserted into the pACTIN vector (63), a gift from Dr Paul Wade, using the Hpa I and Xba I sites. .. To construct pACTINFL-Nrf-1, Nrf-1 was PCR-amplified from a pC MVtag2b vector containing Nrf-1 inserted at the BamHI and Hind III sites (pCMV-Nrf-1) using 50 -TCTGGATCCATGGAGGAACACGGAGTG ACCCAAACCGAA-30 and 50-C GACTCGAGTCACTGTTCCAATGTCACCACCTCC-30.

    Cloning:

    Article Title: Occupancy and synergistic activation of the FMR1 promoter by Nrf-1 and Sp1 in vivo.
    Article Snippet: .. To construct pACTINFL, the FLAG tag and part of the multiple cloning site were PCR-amplified from pCMV-tag2b (Promega) using 50-CGGTTAACGCCACCATGGATTACAAGGATGACG-30 and 50-CCGTCTAGACTTGATATCGAATTCCTGCAGCCC -30 and inserted into the pACTIN vector (63), a gift from Dr Paul Wade, using the Hpa I and Xba I sites. .. To construct pACTINFL-Nrf-1, Nrf-1 was PCR-amplified from a pC MVtag2b vector containing Nrf-1 inserted at the BamHI and Hind III sites (pCMV-Nrf-1) using 50 -TCTGGATCCATGGAGGAACACGGAGTG ACCCAAACCGAA-30 and 50-C GACTCGAGTCACTGTTCCAATGTCACCACCTCC-30.

    Polymerase Chain Reaction:

    Article Title: Occupancy and synergistic activation of the FMR1 promoter by Nrf-1 and Sp1 in vivo.
    Article Snippet: .. To construct pACTINFL, the FLAG tag and part of the multiple cloning site were PCR-amplified from pCMV-tag2b (Promega) using 50-CGGTTAACGCCACCATGGATTACAAGGATGACG-30 and 50-CCGTCTAGACTTGATATCGAATTCCTGCAGCCC -30 and inserted into the pACTIN vector (63), a gift from Dr Paul Wade, using the Hpa I and Xba I sites. .. To construct pACTINFL-Nrf-1, Nrf-1 was PCR-amplified from a pC MVtag2b vector containing Nrf-1 inserted at the BamHI and Hind III sites (pCMV-Nrf-1) using 50 -TCTGGATCCATGGAGGAACACGGAGTG ACCCAAACCGAA-30 and 50-C GACTCGAGTCACTGTTCCAATGTCACCACCTCC-30.

    Plasmid Preparation:

    Article Title: Occupancy and synergistic activation of the FMR1 promoter by Nrf-1 and Sp1 in vivo.
    Article Snippet: .. To construct pACTINFL, the FLAG tag and part of the multiple cloning site were PCR-amplified from pCMV-tag2b (Promega) using 50-CGGTTAACGCCACCATGGATTACAAGGATGACG-30 and 50-CCGTCTAGACTTGATATCGAATTCCTGCAGCCC -30 and inserted into the pACTIN vector (63), a gift from Dr Paul Wade, using the Hpa I and Xba I sites. .. To construct pACTINFL-Nrf-1, Nrf-1 was PCR-amplified from a pC MVtag2b vector containing Nrf-1 inserted at the BamHI and Hind III sites (pCMV-Nrf-1) using 50 -TCTGGATCCATGGAGGAACACGGAGTG ACCCAAACCGAA-30 and 50-C GACTCGAGTCACTGTTCCAATGTCACCACCTCC-30.



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    flag tag and part of the multiple cloning site  (Promega)
    90
    Promega flag tag and part of the multiple cloning site
    Flag Tag And Part Of The Multiple Cloning Site, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag+tag+and+part+of+the+multiple+cloning+site/flag+tag+and+part+of+the+multiple+cloning+site/pm15175277-204-11-17
    Average 90 stars, based on 1 article reviews
    flag tag and part of the multiple cloning site - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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